葡萄球菌α毒素对兔肺泡巨噬细胞的毒性作用
Toxicity of staphylococcal alpha toxin for rabbit alveolar macrophages.
作者信息
McGee M P, Kreger A, Leake E S, Harshman S
出版信息
Infect Immun. 1983 Jan;39(1):439-44. doi: 10.1128/iai.39.1.439-444.1983.
Abstract
Highly purified staphylococcal alpha toxin was toxic in vitro for rabbit alveolar macrophages. Cytotoxicity, manifested by loss of the ability to exclude trypan blue dye and by morphological evidence of cell necrosis and lysis, was observed after exposure for 4 h to 1 microgram of toxin preparation per ml and after exposure for 8 h to 0.1 microgram of toxin per ml. In addition, exposure to toxin under conditions which did not kill more than 10% of the cells (1 microgram/ml for 1.5 to 2 h) significantly reduced the phagocytic activity of the cells and their ability to respond to an activator of hexose monophosphate shunt activity.
摘要
高度纯化的葡萄球菌α毒素在体外对兔肺泡巨噬细胞具有毒性。在每毫升暴露于1微克毒素制剂4小时以及每毫升暴露于0.1微克毒素8小时后，观察到细胞毒性，表现为排除台盼蓝染料的能力丧失以及细胞坏死和裂解的形态学证据。此外，在不杀死超过10%细胞的条件下（1微克/毫升，暴露1.5至2小时）暴露于毒素，会显著降低细胞的吞噬活性及其对己糖磷酸分流活性激活剂的反应能力。
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