通过酶联免疫吸附测定法（COMPELISA）检测补体结合。
Detection of complement fixation by enzyme linked immunosorbant assay (COMPELISA).
作者信息
Hinchliffe P M, Robertson L
出版信息
J Clin Pathol. 1983 Jan;36(1):100-3. doi: 10.1136/jcp.36.1.100.
A method is described by which complement fixation is detected with an enzyme linked immunosorbant assay (ELISA) technique. The method obviates the need for sensitised sheep red blood cells as an indicator of complement fixation and the titration of complement is not critical. The dose response curve has the advantage of being steep and the test result is read photometrically. As test serum and complement do not react together serum anticomplementary effects are eliminated. The ELISA complement fixation test (COMPELISA) was more sensitive than the conventional CFT for detecting brucella antibodies.
本文描述了一种利用酶联免疫吸附测定（ELISA）技术检测补体结合的方法。该方法无需使用致敏绵羊红细胞作为补体结合的指示物，补体的滴定也不关键。剂量反应曲线具有陡峭的优点，并且测试结果通过光度法读取。由于测试血清和补体不一起反应，血清的抗补体作用得以消除。ELISA补体结合试验（COMPELISA）在检测布鲁氏菌抗体方面比传统的补体结合试验（CFT）更敏感。
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