从海洋样本中鉴定副溶血性弧菌的血清学方法。
Serological method for identification of Vibrio parahaemolyticus from marine samples.
作者信息
Shinoda S, Nakahara N, Ninomiya Y, Itoh K, Kane H
出版信息
Appl Environ Microbiol. 1983 Jan;45(1):148-52. doi: 10.1128/aem.45.1.148-152.1983.
Use of agglutination with antiserum against lateral flagella (H-agglutination) for the identification of Vibrio parahaemolyticus was studied. Sucrose-negative bacteria were isolated from seawater, and their characterization was carried out by traditional biological tests and slide agglutination with antiserum specific to lateral flagella of V. parahaemolyticus. Of 135 strains isolated, 78 were identified as V. parahaemolyticus by biological tests and were agglutinated with the above serum. Fifty-five strains did not agglutinate with the serum, and their biological characteristics were different from those of V. parahaemolyticus. Two strains also differed from V. parahaemolyticus in some biological characteristics but agglutinated with the antiserum. All clinically isolated V. parahaemolyticus strains also agglutinated with the above serum. These results suggest that our serological method is useful for the identification of V. parahaemolyticus, especially for samples in which there are many organisms related to V. parahaemolyticus, because many biological tests can be omitted.
研究了使用抗侧鞭毛抗血清的凝集反应（H-凝集反应）来鉴定副溶血性弧菌。从海水中分离出蔗糖阴性细菌，并通过传统生物学试验以及用副溶血性弧菌侧鞭毛特异性抗血清进行玻片凝集试验对其进行鉴定。在分离出的135株菌株中，有78株通过生物学试验鉴定为副溶血性弧菌，并与上述血清发生凝集反应。55株菌株不与该血清凝集，其生物学特性与副溶血性弧菌不同。有两株菌株在某些生物学特性上也与副溶血性弧菌不同，但与抗血清发生凝集反应。所有临床分离的副溶血性弧菌菌株也都与上述血清发生凝集反应。这些结果表明，我们的血清学方法对于副溶血性弧菌的鉴定是有用的，特别是对于存在许多与副溶血性弧菌相关生物的样本，因为可以省略许多生物学试验。
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