Effects of two radiocontrast dyes on the detection of oligoclonal gamma globulins by high resolution agarose gel electrophoresis.

The authors examined two radiocontrast dyes (iophendylate and metrizamide) for their effects on a high resolution agarose electrophoresis procedure for the detection of oligoclonal gamma globulins. Each dye was incubated with cerebrospinal fluid (CSF) and evaluated for effects on sample concentration and on protein migration. Iophendylate interfered with sample concentration but could be removed from CSF by centrifugation. Neither dye appeared to alter protein migration.