使用喹啉-1和喹啉-2探针及荧光显微镜观察单细胞内的pH值和游离钙变化。

Intracellular pH and free calcium changes in single cells using quene 1 and quin 2 probes and fluorescence microscopy.

作者信息

Rogers J, Hesketh T R, Smith G A, Beaven M A, Metcalfe J C, Johnson P, Garland P B

出版信息

FEBS Lett. 1983 Sep 5;161(1):21-7. doi: 10.1016/0014-5793(83)80722-4.

Abstract

Photometric fluorescence microscopy has been used to measure intracellular pH (pHi) and free calcium concentrations [( Ca]i) in individual mouse thymocytes and 2H3 rat basophil leukaemic cells containing indicators for pH (quene 1) or calcium (quin 2). The pHi and [Ca]i measurements in individual 2H3 cells and mouse thymocytes and their responses to various stimuli were consistent with the corresponding data obtained from suspensions of these cells measured in a spectrofluorimeter. Photometric fluorescence microscopy of these indicators in individual cells provides a sensitive and fast method of following pHi and [Ca]i responses in individual cells.

摘要

光度荧光显微镜已被用于测量单个小鼠胸腺细胞和含有pH（喹啉1）或钙（喹啉2）指示剂的2H3大鼠嗜碱性粒细胞白血病细胞内的pH（pHi）和游离钙浓度[（Ca）i]。在单个2H3细胞和小鼠胸腺细胞中进行的pHi和[Ca]i测量及其对各种刺激的反应，与在分光荧光计中测量的这些细胞悬液所获得的相应数据一致。对单个细胞中这些指示剂进行光度荧光显微镜检查，为追踪单个细胞中的pHi和[Ca]i反应提供了一种灵敏且快速的方法。