来自人类染色质的核小体转录
Transcription of nucleosomes from human chromatin.
作者信息
Shaw P A, Sahasrabuddhe C G, Hodo H G, Saunders G F
出版信息
Nucleic Acids Res. 1978 Aug;5(8):2999-3012. doi: 10.1093/nar/5.8.2999.
Nucleosomes (chromatin subunits) prepared by micrococcal nuclease digestion of human nuclei are similar in histone content but substantially reduced in non-histone proteins as compared to undigested chromatin. Chromatin transcription experiments indicate that the DNA in the nucleosomes is accessible to DNA-dependent RNA polymerase in vitro. The template capacities of chromatin and nucleosomes are 1.5 and 10%, respectively, relative to high molecular weight DNA, with intermediate values for oligonucleosomes. Three distinct sizes of transcripts, 150, 120 and 95 nucleotides in length, are obtained when nucleosomes are used as templates. However, when nucleosomal DNA is used as a template, the predominant size of transcripts is 150 nucleotides. When oligonucleosomes are used as templates longer transcripts are obtained. This indicates that RNA polymerase can transcribe the DNA contained in the nucleosomes.
通过微球菌核酸酶消化人细胞核制备的核小体（染色质亚基），其组蛋白含量相似，但与未消化的染色质相比，非组蛋白显著减少。染色质转录实验表明，核小体中的DNA在体外可被依赖DNA的RNA聚合酶所接近。相对于高分子量DNA，染色质和核小体的模板能力分别为1.5%和10%，寡核小体的值介于两者之间。当使用核小体作为模板时，可获得三种不同大小的转录本，长度分别为150、120和95个核苷酸。然而，当使用核小体DNA作为模板时，转录本的主要大小为150个核苷酸。当使用寡核小体作为模板时，可获得更长的转录本。这表明RNA聚合酶可以转录核小体中包含的DNA。
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