Jones P P, Murphy D B, McDevitt H O
Immunogenetics. 1981;12(3-4):321-37. doi: 10.1007/BF01561674.
The genetic and molecular requirements for cell-surface expression of Ia antigens precipitated by anti-I-E subregion sera have been examined. Inbred mice f the d, k, p, and r haplotypes synthesize and express on their lymphocytes the two I-region products normally found in anti-I-E-subregion immunoprecipitates, E alpha and Ae (E beta). Cells from mice of the b and s haplotypes fail to synthesize E alpha chains but do synthesize Ae chains, which remain in the cytoplasm as partially glycosylated precursors. Cells of the f and q haplotypes fail to synthesize either the Ae or E alpha polypeptide chains, as shown by both genetic complementation tests and analyses of total cell proteins by two-dimensional polyacrylamide gel electrophoresis. The patterns of expression of the intact E alpha: Ae complex are consistent with the theory that both the Ae and E alpha polypeptide chains must be present in the cells for either chain to be expressed in normal amounts on the cell surface. The implications of these observations for the genetics of I-region-controlled functions are discussed.
针对抗I-E亚区血清沉淀的Ia抗原在细胞表面表达的遗传和分子要求进行了研究。具有d、k、p和r单倍型的近交系小鼠在其淋巴细胞上合成并表达抗I-E亚区免疫沉淀物中通常发现的两种I区产物，即Eα和Ae（Eβ）。来自b和s单倍型小鼠的细胞不能合成Eα链，但能合成Ae链，这些Ae链以部分糖基化前体的形式保留在细胞质中。f和q单倍型的细胞既不能合成Ae也不能合成Eα多肽链，这通过遗传互补试验以及二维聚丙烯酰胺凝胶电泳对总细胞蛋白的分析得以证实。完整的Eα:Ae复合物的表达模式与以下理论一致，即Ae和Eα多肽链必须同时存在于细胞中，两条链才能在细胞表面以正常量表达。讨论了这些观察结果对I区控制功能遗传学的意义。
