Weir M P, Lo C W
Proc Natl Acad Sci U S A. 1982 May;79(10):3232-5. doi: 10.1073/pnas.79.10.3232.
We have examined the gap junctional communication properties of cells in the wing imaginal disk of Drosophila, using intracellular injection of the fluorescent dye tracer Lucifer Yellow. The cell-to-cell passage of Lucifer Yellow is restricted at a boundary line that divides the wing disk into halves. We refer to each half as a "communication compartment" because there is a high level of gap junctional exchange within a compartment and much lower exchange between compartments. Comparison of the positions of the compartments with developmental fate maps suggests that cells in one communication compartment give rise to cuticular structures in the anterior half of the adult dorsal mesothorax, and cells in the other compartment contribute to posterior structures. This communication-restriction line appears to be coincident with the line between the anterior and posterior developmental compartments observed in studies of cell lineage. We propose that gap junctional communication restrictions may play a general role in generating or maintaining developmental compartments.
我们利用向细胞内注射荧光染料示踪剂路西法黄，研究了果蝇翅成虫盘细胞的间隙连接通讯特性。路西法黄在细胞间的传递在一条将翅成虫盘分成两半的边界线处受到限制。我们将每一半称为一个“通讯区室”，因为区室内间隙连接的交换水平很高，而区室之间的交换则低得多。将这些区室的位置与发育命运图谱进行比较表明，一个通讯区室中的细胞产生成年背中胸前部的表皮结构，而另一个区室中的细胞则形成后部结构。这条通讯限制线似乎与细胞谱系研究中观察到的前后发育区室之间的线重合。我们提出，间隙连接通讯限制可能在产生或维持发育区室方面发挥普遍作用。
