单克隆OKT3和Leu-2a抗体在靶细胞识别或裂解起始水平对人T细胞细胞毒性的选择性抑制。
Selective inhibition of human T cell cytotoxicity at levels of target recognition or initiation of lysis by monoclonal OKT3 and Leu-2a antibodies.
作者信息
Landegren U, Ramstedt U, Axberg I, Ullberg M, Jondal M, Wigzell H
出版信息
J Exp Med. 1982 May 1;155(5):1579-84. doi: 10.1084/jem.155.5.1579.
Abstract
Out of a panel of seven monoclonal antibodies with affinity for human lymphoid cells, three were shown to prevent cytotoxic T cell activity, whereas none affected natural killer cell activity when applied without complement. Anti-OKT3 and anti-Leu-2a, with affinity for all T cells and the cytotoxic/suppressive subset, respectively were both shown to inhibit T killing by their interaction with the effector cell. For anti-OKT3, the inhibition remained after free antibody was washed away. Anti-Leu-2a, in contrast, induced a rapidly reversible inhibition. Using a single cell assay, anti-OKT3 was shown to reduce the lytic ability without affecting target cell binding, whereas anti-Leu-2a prevented the effectors from binding target cells.
摘要
在一组对人淋巴细胞具有亲和力的七种单克隆抗体中，有三种被证明可阻止细胞毒性T细胞的活性，而在无补体情况下应用时，没有一种会影响自然杀伤细胞的活性。分别对所有T细胞和细胞毒性/抑制性子集具有亲和力的抗OKT3和抗Leu-2a，均通过与效应细胞相互作用而抑制T细胞杀伤作用。对于抗OKT3，在洗去游离抗体后抑制作用仍然存在。相比之下，抗Leu-2a诱导的抑制作用迅速可逆。使用单细胞试验表明，抗OKT3可降低裂解能力而不影响靶细胞结合，而抗Leu-2a则阻止效应细胞与靶细胞结合。
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