Celio M R, Inagami T
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3897-900. doi: 10.1073/pnas.78.6.3897.
The multiple physiologic functions of angiotensin II(AII) are generally supposed to be mediated by the peptide generated in the blood circulation. In addition to this extracellular mechanism of AII formation, we have obtained immunohistochemical evidence for the intracellular synthesis of AII in the kidney. Rats were perfused with fixative, and paraffin sections of the kidneys were processed with antisera against renin (EC 3.4.99.19), AII, and other components of the renin--angiotensin system. Renin immunoreactivity was regularly observed in the epithelioid granular cells in the media of the afferent vessel of the glomerulus. AII immunoreactivity was found to coexist within the same cells. This observation points to an intracellular production of AII in the juxtaglomerular epitheloid granular cells. AII may then be released concomitantly with renin in the interstitial fluid and in the blood. The paracrine secretion of AII could exert a local regulatory influence on the tonus of the glomerular vessels.
血管紧张素II（AII）的多种生理功能通常被认为是由血液循环中生成的肽介导的。除了这种AII形成的细胞外机制外，我们还获得了肾脏中AII细胞内合成的免疫组织化学证据。用固定剂灌注大鼠，并用抗肾素（EC 3.4.99.19）、AII和肾素 - 血管紧张素系统其他成分的抗血清处理肾脏石蜡切片。在肾小球入球小动脉中膜的上皮样颗粒细胞中经常观察到肾素免疫反应性。发现AII免疫反应性在同一细胞内共存。这一观察结果表明在肾小球旁上皮样颗粒细胞中有AII的细胞内产生。然后AII可能与肾素一起释放到间质液和血液中。AII的旁分泌分泌可能对肾小球血管的张力产生局部调节作用。
Zotero 插件