嗜水气单胞菌积累细胞外溶血素和蛋白酶的培养基。
Medium for the accumulation of extracellular hemolysin and protease by Aeromonas hydrophila.
作者信息
Riddle L M, Graham T E, Amborski R L
出版信息
Infect Immun. 1981 Sep;33(3):728-33. doi: 10.1128/iai.33.3.728-733.1981.
Abstract
A medium for the accumulation of extracellular hemolysin (300 to 1,600 hemolytic units per ml) and protease (2 to 3 proteolytic units per ml) was developed for an anaerogenic strain of Aeromonas hydrophila. In this medium, growth yields were less but levels of accumulated toxin were greater or equivalent when compared with the same responses in brain heart infusion and nutrient broths. The medium was considered to be partially defined since the conditions for maximum observed hemolysin accumulation (1,600 hemolysin units per ml) were not identified. The results showed that iron and zinc contributed to the control of the extracellular accumulation of both toxins. Whereas iron exerted an inhibitory effect, zinc stimulated the accumulation of both toxins.
摘要
已为嗜水气单胞菌的非产气菌株开发出一种用于积累细胞外溶血素（每毫升300至1600溶血单位）和蛋白酶（每毫升2至3蛋白水解单位）的培养基。在这种培养基中，与脑心浸液和营养肉汤中的相同反应相比，生长产量较低，但毒素积累水平更高或相当。该培养基被认为是部分限定的，因为尚未确定观察到最大溶血素积累（每毫升1600溶血单位）的条件。结果表明，铁和锌有助于控制两种毒素的细胞外积累。铁发挥抑制作用，而锌则刺激两种毒素的积累。
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