Martinez-Hernandez A, Gay S, Miller E J
J Cell Biol. 1982 Feb;92(2):343-9. doi: 10.1083/jcb.92.2.343.
Antibodies specific for the alpha 1 (V) chain and native collagen molecules containing the alpha 1 (V) chain have been used in electron immunohistochemical studies of rat kidney to determine the ultrastructural distribution of this class of collagen molecules. In addition, antibodies against type I collagen and whole basement membrane were used as markers for interstitial collagen and authentic basement membranes. Our results indicate that type V collagen is present in the renal interstitium in different forms: in close apposition to interstitial collagen fibers; in the stromal aspect of vascular basement membranes; and as particulate material not bound to other structures. On the basis of these findings, we postulate a binding or connecting function for this collagen type.
针对α1(V)链以及含有α1(V)链的天然胶原分子的抗体已被用于大鼠肾脏的电子免疫组织化学研究,以确定这类胶原分子的超微结构分布。此外,抗I型胶原和全层基底膜的抗体被用作间质胶原和真实基底膜的标志物。我们的结果表明,V型胶原以不同形式存在于肾间质中:与间质胶原纤维紧密相邻;在血管基底膜的基质面;以及作为未与其他结构结合的颗粒物质。基于这些发现,我们推测这种胶原类型具有结合或连接功能。