一种与鸡脑细胞和肌管表面结合的单克隆抗体：细胞选择性及抗原特性
A monoclonal antibody which binds to the surface of chick brain cells and myotubes: cell selectivity and properties of the antigen.
作者信息
Lemmon V, Staros E B, Perry H E, Gottlieb D I
出版信息
Brain Res. 1982 Mar;255(3):349-60. doi: 10.1016/0165-3806(82)90003-7.
A monoclonal antibody has been obtained which binds to the cell surface of cultured chick myotubes and retinal and tectal neurons but not fibroblasts, myoblasts and embryonic liver cells. Indirect immunocytochemistry reveals that antigen is present in all layers of the chick neural retina. The antibody therefore recognizes an antigen common to most, if not all, chick neurons. The antigen has been identified by staining SDS gels with [125I]monoclonal antibody and appears to be a polydisperse collection of polypeptides with molecular weights centered about 250 kdalton.
已获得一种单克隆抗体，它能与培养的鸡肌管、视网膜及视顶盖神经元的细胞表面结合，但不能与成纤维细胞、成肌细胞和胚胎肝细胞结合。间接免疫细胞化学显示，该抗原存在于鸡神经视网膜的所有层中。因此，该抗体识别的是一种大多数（即便不是全部）鸡神经元共有的抗原。通过用[125I]单克隆抗体对SDS凝胶进行染色，已鉴定出该抗原，它似乎是一组多分散的多肽，分子量集中在250千道尔顿左右。
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