McGrath M H, Hundahl S A
Plast Reconstr Surg. 1982 Jun;69(6):975-85. doi: 10.1097/00006534-198206000-00012.
The number and distribution of myofibroblasts in porcine excisional wounds have been measured over a 16-week period by immunoperoxidase labeling of the smooth-muscle antigen in the cytoplasm of the contractile fibroblasts. Changes in the number of myofibroblasts over time correlate with the rate of wound contraction, and the myofibroblasts were distributed throughout the granulation tissue. These findings support the proposal that the contractile fibroblast is the agent of wound contraction. Significantly fewer myofibroblasts are found near the base of the wound and a larger number of myofibroblasts are found in close proximity to inflammatory foci, suggesting a causal relationship between inflammation and the acquisition of contractile properties by the wound fibroblast. There is evidence of a slightly lower percentage of myofibroblasts in areas of rapid fibroblast replication, and the percentage of myofibroblasts does not vary with changes in the tension across a wound. The immunoperoxidase-staining technique permits the identification of individual myofibroblasts by light microscopy and will be a useful tool for further studies of myofibroblast activity and control.
在16周的时间里，通过对收缩性成纤维细胞质中平滑肌抗原进行免疫过氧化物酶标记，测定了猪切除伤口中肌成纤维细胞的数量和分布。肌成纤维细胞数量随时间的变化与伤口收缩速率相关，且肌成纤维细胞分布于整个肉芽组织中。这些发现支持了收缩性成纤维细胞是伤口收缩介质这一观点。在伤口底部附近发现的肌成纤维细胞明显较少，而在炎症灶附近发现的肌成纤维细胞数量较多，这表明炎症与伤口成纤维细胞获得收缩特性之间存在因果关系。有证据表明，在成纤维细胞快速复制的区域，肌成纤维细胞的百分比略低，并且肌成纤维细胞的百分比不会随伤口张力的变化而改变。免疫过氧化物酶染色技术可通过光学显微镜识别单个肌成纤维细胞，将成为进一步研究肌成纤维细胞活性和调控的有用工具。
