人乳中的维生素D代谢物。

Vitamin D metabolites in human milk.

作者信息

Weisman Y, Bawnik J C, Eisenberg Z, Spirer Z

出版信息

J Pediatr. 1982 May;100(5):745-8. doi: 10.1016/s0022-3476(82)80575-1.

Abstract

The concentrations of unconjugated 25-OHD, 24, 25(OH)2D, and 1,25(OH)2D were measured in human milk by competitive protein-binding radioassays following successive preparative Sephadex LH-20 chromatography and HPLC. The mean (+/- SE) concentration of 25-OHD was 0.37 +/- 0.03 ng/ml, of 24,25(OH)2D was 24.8 +/- 1.9 pg/ml, and of 1,25(OH)2D was 2.2 +/-0.1 pg/ml. The concentration of 25-OHD3 in milk as determined by HPLC and UV detection at 254 nm was 0.27 +/- 0.08 ng/ml. The milk concentrations of vitamin D metabolites did not correlate with the maternal serum 25-OHD levels. The total amounts of unconjugated vitamin D metabolites correspond to the known low bioassayable vitamin D antirachitic activity in human milk.

摘要

在连续进行制备性葡聚糖凝胶LH - 20柱色谱和高效液相色谱后，采用竞争性蛋白结合放射分析法测定人乳中未结合的25 - 羟基维生素D、24,25 - 二羟基维生素D和1,25 - 二羟基维生素D的浓度。25 - 羟基维生素D的平均（±标准误）浓度为0.37±0.03 ng/ml，24,25 - 二羟基维生素D为24.8±1.9 pg/ml，1,25 - 二羟基维生素D为2.2±0.1 pg/ml。通过高效液相色谱和254 nm紫外检测测定的乳中25 - 羟基维生素D3浓度为0.27±0.08 ng/ml。乳中维生素D代谢物的浓度与母体血清25 - 羟基维生素D水平无关。未结合的维生素D代谢物总量与已知的人乳中低生物活性维生素D抗佝偻病活性相对应。