Mackillop W J, Stewart S S, Buick R N
Br J Cancer. 1982 Jun;45(6):812-20. doi: 10.1038/bjc.1982.132.
A method is presented for the description of a heterogeneous cell population with respect to the volume and density of its cellular components, based on computer analysis of the cell-volume spectra of density-gradient fractions. Display programmes were developed to produce either a perspective plot or an isofrequency contour plot ("fingerprint") of the two-parameter data. The use of sequential density and velocity gradients permitted the separation and study of the properties of any subpopulation. We describe the results of an analysis of cellular heterogeneity in an ovarian carcinoma cell line and in 2 cases of ascites cells from human ovarian carcinoma. The proliferative state (labelling index) and growth potential (culture clonogenicity) of cells from one malignant ascites have been "mapped" in terms of density/volume parameters. The results are discussed in terms of their impact on the view of human ovarian carcinoma as a stem-cell system.
本文介绍了一种基于对密度梯度组分的细胞体积光谱进行计算机分析,来描述异质细胞群体的细胞成分体积和密度的方法。开发了显示程序,以生成双参数数据的透视图或等频率等高线图(“指纹”)。连续密度梯度和速度梯度的使用允许分离和研究任何亚群的特性。我们描述了对卵巢癌细胞系以及2例人卵巢癌腹水细胞的细胞异质性分析结果。已根据密度/体积参数“绘制”了来自一例恶性腹水的细胞的增殖状态(标记指数)和生长潜力(培养克隆形成能力)。根据这些结果对将人卵巢癌视为干细胞系统的观点的影响进行了讨论。