Fujimori T, Yamanishi Y, Yamatsu K, Tajima T
J Pharmacol Methods. 1982 Mar;7(2):105-13. doi: 10.1016/0160-5402(82)90022-5.
High performance liquid chromatography with electrochemical detection was developed for the determination of serotonin (5-hydroxytryptamine) in rabbit platelet and plasma. A peak from tissue extract (peak a) was identified as serotonin based on retention time on the chromatogram, using a potential that gave limiting current on both a current-potential curve and gas chromatography-mass spectrum, as compared with that of authentic serotonin. For the assay of serotonin, 5-hydroxy-N-methyltryptamine was used as an internal standard and the extraction recovery of the serotonin added to the platelet-rich plasma was almost total. Furthermore, using this highly sensitive method, it has been demonstrated that the release of endogenous serotonin from rabbit platelets occurs during collagen-induced platelet aggregation but not during adenosine diphosphate-induced platelet aggregation. The procedures described should prove to be simple and useful for pharmacological and physiological investigations on the relationship between platelet aggregation and release reaction.
建立了一种采用电化学检测的高效液相色谱法，用于测定兔血小板和血浆中的5-羟色胺（血清素）。根据色谱图上的保留时间，利用在电流-电位曲线和气相色谱-质谱上产生极限电流的电位，将组织提取物中的一个峰（峰a）鉴定为血清素，并与标准血清素进行比较。在血清素测定中，使用5-羟-N-甲基色胺作为内标，添加到富含血小板血浆中的血清素提取回收率几乎是完全的。此外，使用这种高灵敏度方法已证明，兔血小板内源性血清素的释放发生在胶原诱导的血小板聚集过程中，而不是在二磷酸腺苷诱导的血小板聚集过程中。所述方法对于血小板聚集与释放反应之间关系的药理和生理研究应证明是简单且有用的。
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