多脱氧核苷酸对真核生物DNA聚合酶α的抑制作用。

Inhibition of eukaryotic DNA polymerase-alpha by polydeoxynucleotides.

作者信息

Oguro M, Nagano H

出版信息

J Biochem. 1982 Aug;92(2):599-602. doi: 10.1093/oxfordjournals.jbchem.a133969.

DOI:10.1093/oxfordjournals.jbchem.a133969

Abstract

Effects of polydeoxynucleotides on the activity of DNA polymerase-alpha from sea urchin embryos were studied. Poly(dG), poly(dC) and poly(dC)-oligo(dG)12-18 inhibited DNA polymerase-alpha activity in the activated DNA-directed reaction but poly(dA), oligo(dT)12-18, and poly(dA)-oligo(dT)12-18 did not inhibit the activity. The inhibitory mode of poly(dC)-oligo(dG)12-18 or poly(dC) was competitive with activated DNA and that of poly(dG) was noncompetitive with activated DNA. Using poly(dA)-oligo(dT)12-18 as a template-primer, the inhibition with either poly(dG) or poly(dC)-oligo(dG)12-18 was competitive with the template-primer. These kinetic results indicate that each of the template-primers tested binds to an identical site on DNA polymerase-alpha. Similar results were obtained with DNA polymerase-alpha from HeLa cells.

摘要

研究了多脱氧核苷酸对海胆胚胎DNA聚合酶α活性的影响。聚（dG）、聚（dC）和聚（dC）-寡聚（dG）12-18在活化的DNA指导反应中抑制DNA聚合酶α活性，但聚（dA）、寡聚（dT）12-18和聚（dA）-寡聚（dT）12-18不抑制该活性。聚（dC）-寡聚（dG）12-18或聚（dC）的抑制模式与活化DNA具有竞争性，而聚（dG）的抑制模式与活化DNA不具有竞争性。以聚（dA）-寡聚（dT）12-18作为模板引物，聚（dG）或聚（dC）-寡聚（dG）12-18的抑制作用与模板引物具有竞争性。这些动力学结果表明，所测试的每种模板引物都与DNA聚合酶α上的同一位点结合。从HeLa细胞中获得的DNA聚合酶α也得到了类似的结果。