米帕林（阿的平）对血小板磷脂酶A2活性和血小板聚集的双相调节作用

Biphasic modulation of platelet phospholipase A2 activity and platelet aggregation by mepacrine (quinacrine).

作者信息

Chan A C, Pritchard E T, Gerrard J M, Man R Y, Choy P C

出版信息

Biochim Biophys Acta. 1982 Oct 14;713(1):170-2. doi: 10.1016/0005-2760(82)90179-5.

Abstract

The modulation of rat platelet phospholipase A2 (phosphatide 2-acylhydrolase, EC 3.1.1.4) and rat platelet aggregation by mepacrine was investigated. The 2-acyl specificity of phospholipase A2 activity was confirmed by using 1-[14C]palmitoyl-2-[3H]arachidonylphosphatidylcholine as substrate. Under optimal pH, phospholipase A2 activity was not affected by aspirin but was inhibited by indomethacin. Contrary to previous reports, a biphasic modulatory role of mepacrine on phospholipase A2 activity and platelet aggregation was demonstrated. The data suggest that platelet aggregation is mediated via phospholipase A2.

摘要

研究了米帕林对大鼠血小板磷脂酶A2（磷脂2-酰基水解酶，EC 3.1.1.4）和大鼠血小板聚集的调节作用。以1-[14C]棕榈酰-2-[3H]花生四烯酰磷脂酰胆碱为底物，证实了磷脂酶A2活性的2-酰基特异性。在最佳pH值下，磷脂酶A2活性不受阿司匹林影响，但受吲哚美辛抑制。与先前的报道相反，米帕林对磷脂酶A2活性和血小板聚集具有双相调节作用。数据表明血小板聚集是通过磷脂酶A2介导的。