大鼠肝脏和红细胞中神经节苷脂的结构研究。

Structural study on gangliosides from rat liver and erythrocytes.

作者信息

Rokukawa C, Kushi Y, Ueno K, Handa S

出版信息

J Biochem. 1982 Nov;92(5):1481-8. doi: 10.1093/oxfordjournals.jbchem.a134072.

DOI:10.1093/oxfordjournals.jbchem.a134072

Abstract

Gangliosides were isolated from rat liver and erythrocytes by chromatography on columns of DEAE-Sephadex and Iatrobeads, and finally purified by preparative TLC. The chemical structures of the purified components were studied by carbohydrate analysis, methylation analysis, sialidase treatment, fatty acid analysis and direct mass spectrometry. In rat liver, gangliosides GM3, GM1, GD3, GD1a, GD1b, and GT1b were identified. Gangliosides in rat erythrocytes were characterized as GM1, fucosyl-GM1, and GD1a. Sialic acid was the N-acetyl type only and lignoceric acid was the main fatty acid in all components of rat liver and erythrocytes.

摘要

通过在DEAE-葡聚糖凝胶和离子交换树脂柱上进行色谱分离，从大鼠肝脏和红细胞中分离出神经节苷脂，最后通过制备型薄层色谱法进行纯化。通过碳水化合物分析、甲基化分析、唾液酸酶处理、脂肪酸分析和直接质谱法研究了纯化成分的化学结构。在大鼠肝脏中，鉴定出神经节苷脂GM3、GM1、GD3、GD1a、GD1b和GT1b。大鼠红细胞中的神经节苷脂特征为GM1、岩藻糖基-GM1和GD1a。唾液酸仅为N-乙酰型，二十四酸是大鼠肝脏和红细胞所有成分中的主要脂肪酸。