Piletz J E, Herschman H R
Biochem Genet. 1982 Dec;20(11-12):1221-33. doi: 10.1007/BF00498945.
Adult lethal milk (lm/lm) mutant mice display increased induction of hepatic metallothionein synthesis compared to wild-type mice following the subcutaneous injection of 40 mumol ZnCl2/kg mouse. At this zinc dose the rate of incorporation of [35S] cysteine into hepatic metallothionein in adult (100- to 230-day-old) lm/lm mice was approximately 2.4-fold greater than the rate of incorporation of isotope in wild-type animals. At a higher zinc dose (160 mumol ZnCl2/kg) the incorporation of [35S] cysteine into hepatic metallothionein was similar in lm/lm and wild-type mice. The altered dose-response to zinc administration was not due to a change in hepatic zinc, copper, or manganese levels, to a difference in 65Zn uptake, or to an alteration in 65Zn bound to differential centrifugation fractions of adult lm/lm liver. 65Zn bound to hepatic metallothionein was, however, increased in aging lm/lm mice with symptomatic skin lesions.
与野生型小鼠相比，成年致死性乳鼠（lm/lm）突变小鼠在皮下注射40 μmol ZnCl2/kg小鼠后，肝脏金属硫蛋白合成的诱导增加。在此锌剂量下，成年（100至230日龄）lm/lm小鼠肝脏中[35S]半胱氨酸掺入金属硫蛋白的速率比野生型动物中同位素掺入速率大约高2.4倍。在更高的锌剂量（160 μmol ZnCl2/kg）下，lm/lm小鼠和野生型小鼠肝脏中[35S]半胱氨酸掺入金属硫蛋白的情况相似。锌给药剂量反应的改变并非由于肝脏锌、铜或锰水平的变化、65Zn摄取的差异或成年lm/lm肝脏差速离心组分中结合的65Zn的改变。然而，在有症状性皮肤病变且衰老的lm/lm小鼠中，与肝脏金属硫蛋白结合的65Zn增加。
