一种新的倍半萜抗生素、庚特酸产生菌、发酵、分离及特性研究。

A new sesquiterpene antibiotic, heptelidic acid producing organisms, fermentation, isolation and characterization.

作者信息

Itoh Y, Kodama K, Furuya K, Takahashi S, Haneishi T, Takiguchi Y, Arai M

出版信息

J Antibiot (Tokyo). 1980 May;33(5):468-73. doi: 10.7164/antibiotics.33.468.

DOI:10.7164/antibiotics.33.468

PMID:7191847

Abstract

A new sesquiterpene antibiotic, heptelidic acid, was found in the culture filtrate of three different strains of fungi isolated from soil samples. These strains were identified as Gliocladium virens, Chaetomium globosum and Trichoderma viride. Heptelidic acid was produced by conventional submerged culture and purified by successive column chromatography on silica gel and Sephadex LH-20 and finally by preparative TLC on silica gel. The molecular formula of heptelidic acid was determined as C15H20O5 on the basis of elementary analysis and high resolution mass spectrometry of its monomethyl ester. The antimicrobial spectrum of the antibiotic revealed its specific activity against anaerobic bacteria, especially against Bacteroides fragilis.

摘要

从土壤样本中分离出的三种不同真菌菌株的培养滤液中发现了一种新的倍半萜抗生素——庚特灵酸。这些菌株被鉴定为绿粘帚霉、球毛壳菌和绿色木霉。庚特灵酸通过传统的深层培养产生，并通过在硅胶和葡聚糖凝胶LH - 20上连续柱色谱法纯化，最后通过硅胶制备薄层层析法纯化。基于其单甲酯的元素分析和高分辨率质谱，确定庚特灵酸的分子式为C15H20O5。该抗生素的抗菌谱显示其对厌氧菌具有特异性活性，尤其是对脆弱拟杆菌。