非酶蛋白磷酸化。酰基磷酸对6-磷酸葡萄糖酸脱氢酶的磷酸化作用。
Non-enzymic protein phosphorylation. Phosphorylation of 6-phosphogluconate dehydrogenase by acyl phosphates.
作者信息
Dallocchio F, Matteuzzi M, Bellini T
出版信息
Biochem J. 1982 May 1;203(2):401-4. doi: 10.1042/bj2030401.
Abstract
Incubation of 6-phosphogluconate dehydrogenase from Candida utilis with either acetyl phosphate, 1,3-diphosphoglycerate or carbamoyl phosphate results in the phosphorylation of the protein. The binding of one phosphate residue per enzyme subunit does not affect significantly the kinetic properties, but makes the enzyme less reactive toward thiol reagents, trypsin and pyridoxal 5'-phosphate. We suggest indicate that: (1) 6-phosphogluconate dehydrogenase from C. utilis is phosphorylated non-enzymically by physiological acyl phosphates and (2) the phosphorylation of the enzyme modifies the rate of protein inactivation.
摘要
将产朊假丝酵母的6-磷酸葡萄糖酸脱氢酶与乙酰磷酸、1,3-二磷酸甘油酸或氨基甲酰磷酸一起孵育会导致该蛋白质发生磷酸化。每个酶亚基结合一个磷酸残基不会显著影响其动力学性质，但会使该酶对硫醇试剂、胰蛋白酶和磷酸吡哆醛的反应性降低。我们表明：（1）产朊假丝酵母的6-磷酸葡萄糖酸脱氢酶被生理性酰基磷酸非酶促磷酸化，以及（2）该酶的磷酸化改变了蛋白质失活的速率。
相似文献
1
Non-enzymic protein phosphorylation. Phosphorylation of 6-phosphogluconate dehydrogenase by acyl phosphates.非酶蛋白磷酸化。酰基磷酸对6-磷酸葡萄糖酸脱氢酶的磷酸化作用。
Biochem J. 1982 May 1;203(2):401-4. doi: 10.1042/bj2030401.
2
Evidence for the proximity of a cysteine and a lysine residue in the active site of 6-phosphogluconate dehydrogenase from Candida utilis.
Ital J Biochem. 1983 Mar-Apr;32(2):124-30.
3
Alkylation of 6-phosphogluconate dehydrogenase from Candida utilis with coenzyme analogues.用辅酶类似物对产朊假丝酵母的6-磷酸葡萄糖酸脱氢酶进行烷基化
Eur J Biochem. 1978 Aug 1;88(2):433-8. doi: 10.1111/j.1432-1033.1978.tb12466.x.
4
Overall kinetic mechanism of 6-phosphogluconate dehydrogenase from Candida utilis.产朊假丝酵母6-磷酸葡萄糖酸脱氢酶的整体动力学机制
Biochemistry. 1993 Mar 2;32(8):2036-40. doi: 10.1021/bi00059a021.
5
The active site of 6-phosphogluconate dehydrogenase. A phosphate binding site and its surroundings.
Arch Biochem Biophys. 1978 Aug;189(2):516-23. doi: 10.1016/0003-9861(78)90241-2.
6
The 2'-phosphate of NADP is critical for optimum productive binding to 6-phosphogluconate dehydrogenase from Candida utilis.NADP的2'-磷酸对于与产朊假丝酵母的6-磷酸葡萄糖酸脱氢酶实现最佳有效结合至关重要。
Arch Biochem Biophys. 1993 Sep;305(2):551-8. doi: 10.1006/abbi.1993.1460.
7
Substrate-induced intramolecular proton transfer in 6-phosphogluconate dehydrogenase from Candida utilis.产朊假丝酵母6-磷酸葡萄糖酸脱氢酶中底物诱导的分子内质子转移
Biochim Biophys Acta. 1981 Nov 13;662(1):163-4. doi: 10.1016/0005-2744(81)90238-2.
8
Effect of the substrate on the binding of coenzyme and coenzyme analogues to 6-phosphogluconate dehydrogenase from Candida utilis.底物对辅酶及辅酶类似物与产朊假丝酵母6-磷酸葡萄糖酸脱氢酶结合的影响。
J Biol Chem. 1981 Nov 10;256(21):10778-80.
9
Purification and properties of two forms of 6-phosphogluconate dehydrogenase from Candida utilis.
Eur J Biochem. 1967 Apr;1(2):170-8. doi: 10.1007/978-3-662-25813-2_26.
10
Chemical mechanism of 6-phosphogluconate dehydrogenase from Candida utilis from pH studies.基于pH研究的产朊假丝酵母6-磷酸葡萄糖酸脱氢酶的化学机制
Biochemistry. 1993 Mar 2;32(8):2041-6. doi: 10.1021/bi00059a022.
引用本文的文献
1
Quantitative proteomics analysis of phosphorylated proteins in the hippocampus of Alzheimer's disease subjects.阿尔茨海默病患者海马磷酸化蛋白质的定量蛋白质组学分析。
J Proteomics. 2011 Jun 10;74(7):1091-103. doi: 10.1016/j.jprot.2011.03.033. Epub 2011 Apr 13.
本文引用的文献
1
The effect of inorganic phosphate on the stability of some enzymes.无机磷酸盐对某些酶稳定性的影响。
Biochem J. 1981 Sep 1;197(3):747-9. doi: 10.1042/bj1970747.
2
Enzyme-catalyzed phosphoryl transfer reactions.酶催化的磷酰基转移反应。
Annu Rev Biochem. 1980;49:877-919. doi: 10.1146/annurev.bi.49.070180.004305.
3
Evidences for the involvement of a histidine residue in the binding of the substrate to the 6-phosphogluconate dehydrogenase.关于组氨酸残基参与底物与6-磷酸葡萄糖酸脱氢酶结合的证据。
Arch Biochem Biophys. 1972 Jun;150(2):503-10. doi: 10.1016/0003-9861(72)90068-9.
4
The active site of 6-phosphogluconate dehydrogenase. A phosphate binding site and its surroundings.
Arch Biochem Biophys. 1978 Aug;189(2):516-23. doi: 10.1016/0003-9861(78)90241-2.
5
Evidence for the proximity of two sulfhydryl groups at the active site of 6-phosphogluconate dehydrogenase.
Arch Biochem Biophys. 1978 Mar;186(2):406-10. doi: 10.1016/0003-9861(78)90452-6.
6
6-Phosphogluconate dehydrogenase from Candida utilis.
Methods Enzymol. 1975;41:237-40. doi: 10.1016/s0076-6879(75)41054-0.
7
Phosphorylation-dephosphorylation of enzymes.酶的磷酸化-去磷酸化
Annu Rev Biochem. 1979;48:923-59. doi: 10.1146/annurev.bi.48.070179.004423.
8
Control of rabbit liver fructose-1, 6-diphosphatase activity by magnesium ions.镁离子对兔肝果糖-1,6-二磷酸酶活性的调控
J Biochem. 1975 Dec;78(6):1161-9. doi: 10.1093/oxfordjournals.jbchem.a131012.
Zotero 插件