Lupker J H, Davis A, Jochemsen H, van der Eb A J
J Virol. 1981 Jan;37(1):524-9. doi: 10.1128/JVI.37.1.524-529.1981.
Translation of early RNA specific for the leftmost early region 1 of adenovirus type 5 DNA in a rabbit reticulocyte cell-free system resulted in the synthesis of proteins with the following molecular weights: 65,000 (65K), 54K, 42 to 34K, 29K, 25K, 19K, and 18K. All of these proteins could be immunoprecipitated with hamster antitumor serum. The 42 to 34K proteins mapped in early region 1a, and those with molecular weights of 65K, 19K, and 18K mapped in early region 1b. The gene coding for the 54K protein may be localized outside of early region 1. We could not map unambiguously the 29K and 25K proteins. The identification of a 65K protein among products synthesized in vitro suggests that this protein may be identical to the 65K major T antigen present in adenovirus type 5-infected and -transformed cells, and this indicates that it is indeed encoded by the viral genome. This protein is encoded by a 23S mRNA. The other early region 1-specific proteins appear to be encoded by mRNA of approximately 13S, except for the 19K protein, which is synthesized with RNA sedimenting at both 13S and 23S.
在兔网织红细胞无细胞体系中对腺病毒5型DNA最左侧早期区域1的早期RNA进行翻译，结果合成了分子量如下的蛋白质：65,000（65K）、54K、42至34K、29K、25K、19K和18K。所有这些蛋白质都可用仓鼠抗肿瘤血清进行免疫沉淀。分子量为42至34K的蛋白质定位在早期区域1a，分子量为65K、19K和18K的蛋白质定位在早期区域1b。编码54K蛋白质的基因可能位于早期区域1之外。我们无法明确确定29K和25K蛋白质的定位。在体外合成产物中鉴定出一种65K蛋白质，这表明该蛋白质可能与腺病毒5型感染和转化细胞中存在的65K主要T抗原相同，这表明它确实由病毒基因组编码。该蛋白质由23S mRNA编码。除19K蛋白质外，其他早期区域1特异性蛋白质似乎由约13S的mRNA编码，19K蛋白质由沉降系数为13S和23S的RNA合成。
