Kanai Y, Sugimura T
Immunology. 1981 May;43(1):101-10.
Immunochemical studies were made on the antibodies induced in rabbits against poly(ADP-ribose) and naturally-occurring antibodies in patients with systemic lupus erythematosus. Antibodies against poly(ADP-ribose) could also be induced in rabbits by oligo(ADP-ribose) associated with rat liver histones and by a complex of poly(ADP-ribose) with methylated bovine serum albumin (MBSA). The two types of antibody were inhibited to the same extent by poly(ADP-ribose). However, the antibody induced by oligo(ADP-ribose) associated with histones was inhibited by oligo(ADP-ribose) with an average chain length if 4 ADP-ribosyl units and by phosphoribosyl adenosine monophosphate (PR-AMP) but not by mono ADP-ribose, whereas that induced by poly(ADP-ribose) was practically not inhibited by these related compounds even in excess amounts. The sera of ten cases of systemic lupus erythematosus showing high antibody activity against poly(ADP-ribose) were also examined immunochemically. It was found that the antibodies of three patients showed a similar inhibitory pattern to that of antibody induced in rabbits by oligo(ADP-ribose) associated with histones, those of three patients showed a similar pattern to that of antibody produced in rabbits by poly(ADP-ribose), and the remainder did not show either pattern. These findings suggest that oligo(ADP-ribose) associated with histones may serve as antigen to elicit naturally-occuring antibodies to poly(ADP-ribose) in patients with systemic lupus erythematosus.
对兔体内诱导产生的抗聚(ADP - 核糖)抗体以及系统性红斑狼疮患者体内的天然抗体进行了免疫化学研究。与大鼠肝脏组蛋白相关的寡聚(ADP - 核糖)以及聚(ADP - 核糖)与甲基化牛血清白蛋白(MBSA)的复合物也能在兔体内诱导产生抗聚(ADP - 核糖)抗体。这两种抗体都能被聚(ADP - 核糖)同等程度地抑制。然而,与组蛋白相关的寡聚(ADP - 核糖)诱导产生的抗体能被平均链长为4个ADP - 核糖基单位的寡聚(ADP - 核糖)和磷酸核糖腺苷单磷酸(PR - AMP)抑制,但不能被单磷酸ADP - 核糖抑制,而聚(ADP - 核糖)诱导产生的抗体即使在过量这些相关化合物存在的情况下也几乎不被抑制。还对10例对聚(ADP - 核糖)具有高抗体活性的系统性红斑狼疮患者的血清进行了免疫化学检测。发现3例患者的抗体呈现出与兔体内由与组蛋白相关的寡聚(ADP - 核糖)诱导产生的抗体类似的抑制模式,3例患者的抗体呈现出与兔体内由聚(ADP - 核糖)产生的抗体类似的模式,其余患者则未呈现出这两种模式中的任何一种。这些发现表明,与组蛋白相关的寡聚(ADP - 核糖)可能作为抗原,在系统性红斑狼疮患者体内引发针对聚(ADP - 核糖)的天然抗体。