番泻苷A和B等泻药的高压液相色谱分析。

High-pressure liquid chromatographic analysis of sennosides A and B purgative drugs.

作者信息

Komolafe O O

出版信息

J Pharm Sci. 1981 Jul;70(7):727-30. doi: 10.1002/jps.2600700704.

Abstract

Procedures are described for the analysis of the main anthraquinone glycosides of senna powder, senna fruit tablets, and sennoside tablets by high-pressure liquid chromatography (HPLC). In one HPLC analysis, TLC was used to separate the glycosides prior to elution on a strong anion-exchange column with 0.1 M ammonium nitrate solution (pH 9.0) as the mobile phase. In another HPLC analysis, separation was effected using a weak anion-exchange column with 0.1 M ammonium nitrate solution (pH 5.7A) as the mobile phase.

摘要

本文描述了用高压液相色谱法（HPLC）分析番泻叶粉、番泻果实片和番泻苷片主要蒽醌苷的方法。在一次HPLC分析中，采用薄层色谱法（TLC）在强阴离子交换柱上洗脱之前先分离苷类，流动相为0.1M硝酸铵溶液（pH 9.0）。在另一次HPLC分析中，采用弱阴离子交换柱，流动相为0.1M硝酸铵溶液（pH 5.7A）进行分离。