Rechnitz G A
Science. 1981 Oct 16;214(4518):287-91. doi: 10.1126/science.7280694.
The use of intact bacterial cells or tissue slices of plant and animal origin as immobilized biocatalysts has extended the possible range of potentiometric bioselective membrane electrodes beyond that of conventional enzyme electrodes. The use of such materials as biocatalysts offers advantages in situations where isolated enzymes are not available or where multistep reaction paths are required. The resulting bioselective electrodes also offer exceptional ease of preparation, time stability, and low cost.
使用完整的细菌细胞或动植物来源的组织切片作为固定化生物催化剂,已将电位型生物选择性膜电极的可能范围扩展到超出传统酶电极的范围。在无法获得分离酶或需要多步反应路径的情况下,使用此类材料作为生物催化剂具有优势。由此产生的生物选择性电极还具有制备异常简便、时间稳定性好和成本低的特点。