[Effect on freezing on hydroxylation in isolated hepatocytes].

Rapid freezing of hepatocytes down to--196 degrees was accompanied by a distinct impairment in microsomal oxidation and by complete uncoupling of respiration and oxidative phosphorylation in mitochondria. In the course of multistep freezing activity of the microsomal oxidation system constituted 70.3% with ethylene glycol (EG) and 78% - with dimethyl sulfoxide (DMSO), while the functional activity of mitochondria was maintained by 30% and 40% if EG DMSO were used, respectively.