丙烷喷射冷冻：一种用于保存温度依赖性脂质相的有效超快速冷冻方法。
Propane jet-freezing: a valid ultra-rapid freezing method for the preservation of temperature dependent lipid phases.
作者信息
van Venetië R, Hage W J, Bluemink J G, Verkleij A J
出版信息
J Microsc. 1981 Sep;123(Pt 3):287-92. doi: 10.1111/j.1365-2818.1981.tb02472.x.
We have developed a temperature controlled specimen stage on the existing jet-freezing apparatus, in order to quench samples from elevated temperatures up to 330 K. We have shown that the phase transition both from the liquid-crystalline to solid phase for pure phosphatidylcholines and from hexagonal II to lamellar phase for phosphatidylethanolamine/4% egg phosphatidic acid is prevented. Moreover, cooling rates exceeding 10,000 K s-1 were recorded indicative of ultra-rapid freezing capacity. Cryoprotectants can be omitted, since phase separation due to ice crystal growth does not occur.
我们在现有的喷射冷冻设备上开发了一个温度可控的样品台，以便将样品从高达330 K的高温快速冷却。我们已经证明，纯磷脂酰胆碱从液晶相到固相的相变以及磷脂酰乙醇胺/4% 鸡蛋磷脂酸从六方II相到层状相的相变都得到了抑制。此外，记录到的冷却速率超过10,000 K s-1，表明具有超快速冷冻能力。由于不会发生冰晶生长导致的相分离，因此可以省略冷冻保护剂。
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