[癌前和癌性胃黏膜中乳酸脱氢酶同工酶的测定]

[Determination of lactic dehydrogenase isoenzymes in precancerous and cancerous gastric mucosa].

作者信息

Mazzarino C, Malaponte G, Travali S, Sanfilippo G, Russo A

出版信息

Boll Soc Ital Biol Sper. 1981 Oct 15;57(19):2004-10.

Abstract

We have studied modifications of LDH isoenzymes pattern in normal human gastric mucosa as well as in adenocarcinoma and precancerous lesions of the stomac (gastritis and ulcer); samples from the injured and the surrounding non-injured area were examined, drawing up the isoenzymes, using Tris-buffer pH 7,4 at 4 degrees C and performing the determination within 1 h - because of the high chronolability of the fractions LDH and LDH by cellogol electrophoresis separation. We have always noticed - especially in samples from adenocarcinoma- a shifting toward the M chains, with a clear increase of the fractions LDH4 and LDH5; this has been noticed even in the surrounding non-injured area.

摘要

我们研究了正常人胃黏膜以及胃癌、癌前病变（胃炎和溃疡）中乳酸脱氢酶同工酶模式的变化；对损伤部位及其周围未损伤区域的样本进行了检查，使用pH 7.4的Tris缓冲液在4℃下绘制同工酶，并在1小时内进行测定——因为通过纤维素电泳分离，乳酸脱氢酶各组分的时效性很高。我们一直注意到——尤其是在腺癌样本中——向M链的转移，乳酸脱氢酶4和乳酸脱氢酶5组分明显增加；在周围未损伤区域也观察到了这种情况。