多头绒泡菌5S核糖体RNA的核苷酸序列。

The nucleotide sequence of 5S ribosomal RNA from slime mold Physarum polycephalum.

作者信息

Komiya H, Takemura S

出版信息

J Biochem. 1981 Dec;90(6):1577-81. doi: 10.1093/oxfordjournals.jbchem.a133631.

DOI:10.1093/oxfordjournals.jbchem.a133631

Abstract

The nucleotide sequence of 5S ribosomal RNA from plasmodia of the slime mold Physarum polycephalum was determined as pppGGAUGCGGC CAUACUAAGG 20 AGAAAGCACC 30 UCAUCCCGUC 40 CGAUCUGAGA 50 AGUUAAGCUC 60 CUUCAGGCGU 70 GGUUAGUACU 80 GGGGUGGGGG 90 ACCACCUGGG 100 AAUCCCACGU 110 GCUGCAUUCU 120 Uoh by chemical and enzymatic gel sequencing technics using 3' and 5' end-labeled RNA. This RNA is very different from 5S rRNA of the cellular slime mold Dictyostelium discoideum (36 nucleotides are different), and shows greater similarity to 5S rRNAs from Protozoa and Metazoa than to those from fungi.

摘要

利用3'和5'末端标记的RNA，通过化学和酶促凝胶测序技术确定了多头绒泡菌黏菌疟原虫5S核糖体RNA的核苷酸序列为pppGGAUGCGGC CAUACUAAGG 20 AGAAAGCACC 30 UCAUCCCGUC 40 CGAUCUGAGA 50 AGUUAAGCUC 6GU 70 GGUUAGUACU 80 GGGGUGGGGG 90 ACCACCUGGG 100 AAUCCCACGU 110 GCUGCAUUCU 120 Uoh。这种RNA与细胞黏菌盘基网柄菌的5S rRNA有很大不同（36个核苷酸不同），并且与原生动物和后生动物的5S rRNA的相似性高于与真菌的5S rRNA的相似性。