多层膜分析仪：用于检测血清、血浆、脑脊液及尿液中的葡萄糖；以及血清和血浆中的尿素氮。

The multilayered film analyzer: glucose in serum, plasma, cerebrospinal fluid, and urine; and urea nitrogen in serum and plasma.

作者信息

Warren K, Kubasik N P, Brody B B, Sine H E, D'Souza J P

出版信息

Clin Chem. 1980 Jan;26(1):133-7.

Abstract

Some basic laboratory performance criteria were studied for the Eastman Kodak glucose and urea nitrogen analyzer. Serum, plasma, urine, and cerebrospinal fluid were tested. Precision, both "within-day" and "between-day," for both analytes was less than 2.2% (CV). Both analytes demonstrated linearity, with about 100% recovery of added substrates. Comparisons with continuous-flow procedures demonstrated good correlation. A variety of sera and plasmas can be used for glucose analysis; oxalate--fluoride-treated plasma is unacceptable for use in analysis for urea nitrogen. We saw no effects on glucose or urea nitrogen analysis from hemolysis, lipemia, icterus, some common drugs, ammonia, or abnormally high protein concentrations. Minimum amounts detectable were: glucose (serum) 104 mg/L; glucose (cerebrospinal fluid) 100 mg/L; and urea nitrogen (serum) 21 mg/L. Calibration procedures are discussed for protein-based and aqueous samples.

摘要

对伊士曼柯达葡萄糖和尿素氮分析仪的一些基本实验室性能标准进行了研究。对血清、血浆、尿液和脑脊液进行了检测。两种分析物的“日内”和“日间”精密度均小于2.2%（变异系数）。两种分析物均显示出线性，添加底物的回收率约为100%。与连续流动程序的比较显示出良好的相关性。多种血清和血浆可用于葡萄糖分析；草酸盐-氟化物处理的血浆不可用于尿素氮分析。我们未发现溶血、脂血、黄疸、一些常见药物、氨或异常高蛋白浓度对葡萄糖或尿素氮分析有影响。可检测的最小量为：葡萄糖（血清）104mg/L；葡萄糖（脑脊液）100mg/L；尿素氮（血清）21mg/L。讨论了基于蛋白质的样品和水性样品的校准程序。