Chernoff A, Levine R F, Goodman D S
J Clin Invest. 1980 Apr;65(4):926-30. doi: 10.1172/JCI109747.
Growth factor activity, as determined by the stimulation of [3H]thymidine incorporation into the DNA of quiescent 3T3 cells in culture, was found in lysates of guinea pig platelets and megakaryocytes. Quantitative dilution studies demonstrated that, of the cells present in the guinea pig bone marrow, only the megakaryocyte possessed quantitatively significant growth factor activity. The amount of activity present in one megakaryocyte was equivalent to that present in 1,000-5,000 platelets, a value approximately comparable to the number of platelets shed from a single megakaryocyte. It is suggested that guinea pig platelet-derived growth factor has its origin in the megakaryocyte.
通过刺激[3H]胸腺嘧啶核苷掺入培养的静止3T3细胞的DNA来测定生长因子活性,结果在豚鼠血小板和巨核细胞的裂解物中发现了该活性。定量稀释研究表明,在豚鼠骨髓中的细胞中,只有巨核细胞具有数量上显著的生长因子活性。一个巨核细胞中存在的活性量相当于1000 - 5000个血小板中存在的活性量,该值大约与单个巨核细胞释放的血小板数量相当。提示豚鼠血小板衍生生长因子起源于巨核细胞。