Lindow S E, Arny D C, Upper C D
Appl Environ Microbiol. 1978 Dec;36(6):831-8. doi: 10.1128/aem.36.6.831-838.1978.
A replica plating method for rapid quantitation of ice nucleation-active (INA) bacteria was developed. Leaf washings of plant samples from California, Colorado, Florida, Louisiana, and Wisconsin were tested for the presence of INA bacteria. Of the 95 plant species sampled, 74 were found to harbor INA bacteria. Only the conifers were, as a group, unlikely to harbor INA bacteria. All of the INA bacteria isolated resembled either Pseudomonas syringae or Erwinia herbicola. Sufficient numbers of INA bacteria were present on the samples to account for the ice nuclei associated with leaves that are necessary for freezing injury to occur. Numbers of INA bacteria were large enough to suggest that plant surfaces may constitute a significant source of atmospheric ice nuclei.
开发了一种用于快速定量冰核活性(INA)细菌的影印培养法。对来自加利福尼亚州、科罗拉多州、佛罗里达州、路易斯安那州和威斯康星州的植物样本的叶洗液进行了INA细菌检测。在采样的95种植物物种中,发现74种含有INA细菌。只有针叶树作为一个群体不太可能含有INA细菌。分离出的所有INA细菌都类似于丁香假单胞菌或草生欧文氏菌。样本上存在足够数量的INA细菌,足以解释与叶片相关的冰核,而这些冰核是发生冻害所必需的。INA细菌的数量足够多,表明植物表面可能是大气冰核的重要来源。