根据竞争性放射免疫分析所获数据计算抗半抗原血清中的平均抗体亲和力。

Calculation of average antibody affinity in anti-hapten sera from data obtained by competitive radioimmunoassay.

作者信息

Müller R

出版信息

J Immunol Methods. 1980;34(4):345-52. doi: 10.1016/0022-1759(80)90107-6.

Abstract

A method is described for rapid and precise determination of average antibody affinity in anti-hapten sera from the following data obtained by competitive radioimmunoassay (RIA): the molar hapten (inhibitor) concentration giving 50% inhibition of tracer-antibody binding under equilibrium conditions, the molar tracer concentration in the assay and the amount of tracer bound by the antibodies in the absence of inhibitor. Thus, no additional experiments are required once a competitive RIA has been established. As an example, the average antibody affinity constants for 2'-deoxyguanosine, O6-ethyl-guanosine, and O6-ethyl-2'-deoxyguanosine were determined in antisera raised against the respective nucleoside-protein conjugates.

摘要

本文描述了一种方法，可根据竞争性放射免疫分析（RIA）获得的以下数据，快速、精确地测定抗半抗原血清中的平均抗体亲和力：在平衡条件下使示踪剂 - 抗体结合受到50%抑制的半抗原（抑制剂）摩尔浓度、分析中的示踪剂摩尔浓度以及在无抑制剂情况下抗体结合的示踪剂数量。因此，一旦建立了竞争性RIA，就无需进行额外实验。例如，针对相应核苷 - 蛋白质缀合物产生的抗血清中，测定了2'-脱氧鸟苷、O6 - 乙基 - 鸟苷和O6 - 乙基 - 2'-脱氧鸟苷的平均抗体亲和常数。