高压氧改善兔实验性葡萄球菌骨髓炎的机制。

A mechanism for the amelioration by hyperbaric oxygen of experimental staphylococcal osteomyelitis in rabbits.

作者信息

Mader J T, Brown G L, Guckian J C, Wells C H, Reinarz J A

出版信息

J Infect Dis. 1980 Dec;142(6):915-22. doi: 10.1093/infdis/142.6.915.

DOI:10.1093/infdis/142.6.915

Abstract

Although hyperbaric oxygen (HBO) is as effective as cephalothin against osteomyelitis due to Staphylococcus aureus in the rabbit, the effect is not by directing killing. To investigate the mechanism, argon washouts (perfusion) and oxygen tensions were measured by intramedullary probes placed in the metaphyses of infected and uninfected tibias. In vitro phagocytic killing activity for S. aureus was determined at oxygen tensions found in these bones under ambient and HBO conditions. Mean tibial oxygen tensions (mm Hg) under ambient conditions were 21 (infected) and 45 (uninfected); under HBO conditions, 104 (infected) and 321 (uninfected). Perfusion was decreased in osteomyelitic bone and was not acutely increased by HBO in either normal or infected bone. Phagocytic killing of S. aureus was markedly decreased at 23 mm Hg of O2, significantly improved at 45 and 109 mm Hg, and most effective at 150 mm Hg. Thus, in osteomyelitic bone, HBO increased intramedullary oxygen to tensions consistent with normal phagocytic function.

摘要

虽然高压氧（HBO）在治疗兔金黄色葡萄球菌引起的骨髓炎方面与头孢菌素同样有效，但其作用并非通过直接杀灭细菌。为了研究其机制，通过将髓内探头置于感染和未感染胫骨的干骺端来测量氩洗脱（灌注）和氧张力。在环境条件和高压氧条件下，测定在这些骨骼中发现的氧张力下金黄色葡萄球菌的体外吞噬杀伤活性。环境条件下胫骨平均氧张力（mmHg）为：感染的21，未感染的45；高压氧条件下，感染的104，未感染的321。骨髓炎骨的灌注减少，在正常或感染骨中高压氧均未使其急性增加。金黄色葡萄球菌在23 mmHg O2时吞噬杀伤明显减少，在45和109 mmHg时显著改善，在150 mmHg时最有效。因此，在骨髓炎骨中，高压氧将髓内氧增加到与正常吞噬功能一致的张力。