Vivas I M, Drujan B D
Neurochem Res. 1980 Aug;5(8):817-29. doi: 10.1007/BF00965782.
Acetylcholine (ACh) synthesis and release from isolated superfused retina of the teleost Eugerres plumieri has been studied under different physiological conditions. The retinas were superfused with Krebs-Ringer solutions containing [14C]choline and the extracellular space of 32% was determined by [3H]inulin. The retina accumulates choline (Ch) from the superfusion medium and this process is mediated by a high affinity transport system with a Km of 1.82 microM. The incorporated Ch is mainly utilized for the synthesis of ACh. The ACh content of the light-adapted retina is not significantly different from that of a dark-adapted one. However, the release of [14C]ACh from the light-adapted retina was 52% higher as compared to the release from the dark-adapted retina. Flicker stimulation induced a larger increase in ACh release, than from either light or dark adapted retina, proportional to flicker frequency. The results suggest that changes in ACh utilization were related to the function of cellular units responsible for light changes transduction rather than light detection.
在不同生理条件下，对硬骨鱼尤氏真鯛分离的超灌注视网膜中乙酰胆碱（ACh）的合成与释放进行了研究。视网膜用含有[14C]胆碱的 Krebs-Ringer 溶液进行超灌注，细胞外空间通过[3H]菊粉测定为 32%。视网膜从超灌注培养基中积累胆碱（Ch），这一过程由 Km 为 1.82 microM 的高亲和力转运系统介导。摄入的 Ch 主要用于 ACh 的合成。光适应视网膜的 ACh 含量与暗适应视网膜的 ACh 含量无显著差异。然而，与暗适应视网膜相比，光适应视网膜释放的[14C]ACh 高出 52%。闪烁刺激比光适应或暗适应视网膜引起的 ACh 释放增加更大，且与闪烁频率成正比。结果表明，ACh 利用的变化与负责光变化转导而非光检测的细胞单位功能有关。
