地塞米松诱导内皮细胞产生的血管皮质素样蛋白的特性研究

Characterization of vasocortin-like proteins induced by dexamethasone in endothelial cells.

作者信息

Iuvone T, Carnuccio R, Di Rosa M

机构信息

Department of Experimental Pharmacology, University of Naples, Federico II, Italy.

出版信息

Eur J Pharmacol. 1993 Oct 19;243(2):163-7. doi: 10.1016/0014-2999(93)90376-s.

DOI:10.1016/0014-2999(93)90376-s

PMID:7506215

Abstract

In this study we have shown that dexamethasone induces vasocortin-like proteins in bovine endothelial cells as well as in the bovine aortic endothelial cell line, GM 7373. Vasocortin-like proteins have been characterized by their ability to mimic the glucocorticoid inhibition of rat dextran oedema and histamine release induced by concanavalin-A in rat mast cells. Following partial purification of these proteins by gel filtration, vasocortin activity was found to be associated to proteins with molecular weight between 20-35 kD. This study showed that dexamethasone induces vasocortin-like proteins in endothelial cells and suggests that endothelial cells are target cells of glucocorticoid activity in vascular tissue.

摘要

在本研究中，我们已表明地塞米松可在牛内皮细胞以及牛主动脉内皮细胞系GM 7373中诱导血管皮质素样蛋白。血管皮质素样蛋白的特征在于它们能够模拟糖皮质激素对大鼠葡聚糖水肿的抑制作用以及伴刀豆球蛋白A诱导的大鼠肥大细胞中组胺释放的抑制作用。通过凝胶过滤对这些蛋白进行部分纯化后，发现血管皮质素活性与分子量在20 - 35 kD之间的蛋白相关。本研究表明地塞米松可在内皮细胞中诱导血管皮质素样蛋白，并提示内皮细胞是血管组织中糖皮质激素活性的靶细胞。