在原位杂交条件下DNA链性会发生变化。
DNA strandness changes during in situ hybridization conditions.
作者信息
Peretti D, Mezzanotte R
机构信息
Istituto di Biologia, Facoltà di Medicina, Università di Cagliari, Italy.
出版信息
Eur J Histochem. 1993;37(4):303-8.
PMID:7510536
Abstract
DNA strandness changes occurring during in situ hybridization were monitored using monoclonal antibodies. Our results show that: 1) RNase induces formation of single stranded DNA, 2) after denaturation-renaturation, single stranded DNA is found principally in centromeric areas, and 3) double stranded DNA is still observed after each step.
摘要
使用单克隆抗体监测原位杂交过程中发生的DNA链性变化。我们的结果表明：1）核糖核酸酶诱导单链DNA的形成；2）变性-复性后，单链DNA主要存在于着丝粒区域；3）每一步之后仍可观察到双链DNA。
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