使用针对来自乳糜泻激活结构域I的α-麦醇溶蛋白测序肽段的单克隆抗体来测量麸质。

Measurement of gluten using a monoclonal antibody to a sequenced peptide of alpha-gliadin from the coeliac-activating domain I.

作者信息

Ellis H J, Doyle A P, Wieser H, Sturgess R P, Day P, Ciclitira P J

机构信息

Rayne Institute, St. Thomas' Hospital, London, UK.

出版信息

J Biochem Biophys Methods. 1994 Jan;28(1):77-82. doi: 10.1016/0165-022x(94)90066-3.

DOI:10.1016/0165-022x(94)90066-3

PMID:7512105

Abstract

A monoclonal antibody, raised against a sequenced 54 amino-acid peptide from the coeliac-activating N-terminal region of alpha-gliadin, was used in an assay for the measurement of gluten in foods. A double-sandwich ELISA using a polyclonal capture antibody produced standard curves for unfractionated gliadin and its alpha, beta, gamma and omega subfractions, and for rye, barley and oat prolamins. The sensitivity of the assay for unfractionated gliadin and rye prolamins was 15 ng/ml, for barley and oat prolamins 125 and 250 ng/ml, respectively. Prolamins from coeliac non-toxic rice, maize, millet and sorghum did not cross-react in the assay.

摘要

一种单克隆抗体是针对来自α-麦醇溶蛋白腹腔激活N端区域的一段54个氨基酸的测序肽产生的，用于食品中麸质的检测。使用多克隆捕获抗体的双夹心ELISA法为未分级的麦醇溶蛋白及其α、β、γ和ω亚组分以及黑麦、大麦和燕麦醇溶蛋白生成了标准曲线。该检测方法对未分级麦醇溶蛋白和黑麦醇溶蛋白的灵敏度为15 ng/ml，对大麦和燕麦醇溶蛋白的灵敏度分别为125 ng/ml和250 ng/ml。来自腹腔无毒的大米、玉米、小米和高粱的醇溶蛋白在该检测中不发生交叉反应。