Mattar S, Centella A, Gamboa F, Arrieta S
Universidad Javeriana, Facultad de Ciencias, Departamento de Microbiología, Bogotá, Columbia.
Enferm Infecc Microbiol Clin. 1994 Mar;12(3):123-6.
To evaluate the catalase test as a screening method in urinary tract infection (UTI) versus sediment, reactive strip and Gram.
Two hundred forty-five's stain urine samples were prospectively analyzed in the Hospital Universitario San Ignacio de Bogotá (Colombia). The culture was used as a reference test for the evaluation of the screening parameters in UTI patients.
Of the 245 urine samples 45 were discarded. The remaining 200 urine samples were cultured being 100 positive and 100 negative. The former were analyzed by screening methods. The sensitivity and specificity of the catalase test was 97% and 94%, respectively with a positive predictive value of 94% and negative of 97%. The most frequently isolated microorganism was E. coli (84%), followed by Proteus sp. (6%).
The use of the catalase test in urinary tract infection is a safe, economic and rapid method providing advantages due to its high sensitivity and specificity values, its good correlation with the different parameters evaluated in this study (sediment, strip, Gram's stain), and offers optimum diagnosis in urinary tract infection in developing countries such as Colombia.
评估过氧化氢酶试验作为尿路感染(UTI)筛查方法与尿沉渣、反应性试纸条及革兰氏染色法相比的效果。
对哥伦比亚波哥大圣伊格纳西奥大学医院前瞻性分析的245份尿液样本进行研究。将培养结果用作评估UTI患者筛查参数的参考标准。
245份尿液样本中有45份被丢弃。其余200份尿液样本进行培养,其中100份呈阳性,100份呈阴性。对阳性样本采用筛查方法进行分析。过氧化氢酶试验的敏感性和特异性分别为97%和94%,阳性预测值为94%,阴性预测值为97%。最常分离出的微生物是大肠杆菌(84%),其次是变形杆菌属(6%)。
在尿路感染中使用过氧化氢酶试验是一种安全、经济且快速的方法,因其高敏感性和特异性值、与本研究评估的不同参数(尿沉渣、试纸条、革兰氏染色)具有良好相关性而具有优势,并且为哥伦比亚等发展中国家的尿路感染提供了最佳诊断方法。