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囊性纤维化患者痰液中的生物活性肿瘤坏死因子

Bioactive tumor necrosis factor in the sputa of cystic fibrosis patients.

作者信息

Kelly N, Clee S, Nakielna B

机构信息

Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada.

出版信息

Clin Diagn Lab Immunol. 1995 Jul;2(4):499-502. doi: 10.1128/cdli.2.4.499-502.1995.

DOI:10.1128/cdli.2.4.499-502.1995
PMID:7583934
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC170189/
Abstract

Eighty sputum samples from 37 cystic fibrosis patients were assayed for tumor necrosis factor alpha (TNF-alpha) in the L929 bioactivity assay. In addition, the presence of TNF-alpha-specific mRNA in the sputum was determined by using TNF-alpha-specific DNA probes in a PCR amplification step after reverse transcription of the mRNA to cDNA. Bioactive TNF-alpha was detected in 69 (86%) of the 80 sputum samples tested and, on at least one occasion, in 35 (95%) of the 37 cystic fibrosis patients sampled. The highest levels of TNF-alpha were observed in sputa obtained from patients presenting with an exacerbation of their disease.

摘要

采用L929生物活性分析法对37例囊性纤维化患者的80份痰液样本进行了肿瘤坏死因子α（TNF-α）检测。此外，在将mRNA逆转录为cDNA后的PCR扩增步骤中，使用TNF-α特异性DNA探针来测定痰液中TNF-α特异性mRNA的存在情况。在所检测的80份痰液样本中，有69份（86%）检测到生物活性TNF-α，在至少一次检测中，37例采样的囊性纤维化患者中有35例（95%）检测到生物活性TNF-α。在疾病加重的患者痰液中观察到最高水平的TNF-α。

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本文引用的文献

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Tumor necrosis factor: an updated review of its biology.肿瘤坏死因子：其生物学的最新综述
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Sputum tumour necrosis factor-alpha and leukotriene concentrations in cystic fibrosis.囊性纤维化患者痰液中肿瘤坏死因子-α和白三烯的浓度
Arch Dis Child. 1993 Mar;68(3):389-92. doi: 10.1136/adc.68.3.389.
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Cytokines in sputum and serum from patients with cystic fibrosis and chronic Pseudomonas aeruginosa infection as markers of destructive inflammation in the lungs.囊性纤维化和慢性铜绿假单胞菌感染患者痰液和血清中的细胞因子作为肺部破坏性炎症的标志物。
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Increased levels of interleukin-1 in bronchoalveolar washings from children with bacterial pulmonary infections.患有细菌性肺部感染的儿童支气管肺泡灌洗中白细胞介素-1水平升高。
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