重组甲型肝炎病毒14S和70S亚病毒颗粒的抗原性和免疫原性特性。
Antigenic and immunogenic properties of recombinant hepatitis A virus 14S and 70S subviral particles.
作者信息
Stapleton J T, Raina V, Winokur P L, Walters K, Klinzman D, Rosen E, McLinden J H
机构信息
Research Service, Iowa City VA Medical Center, Iowa.
出版信息
J Virol. 1993 Feb;67(2):1080-5. doi: 10.1128/JVI.67.2.1080-1085.1993.
Hepatitis A virus (HAV) has an immunodominant neutralization antigenic site. By using a panel of monoclonal antibodies targeted against the HAV neutralization antigenic site, it was shown that three epitopes within this site are present on 14S subunits (pentamers of the structural unit). In contrast, two other epitopes within this site are formed upon assembly of 14S subunits into capsids. Thus, the epitopes recognized by these two monoclonal antibodies are formed either by a conformational change in the antigenic site or by the juxtaposition of epitope fragments present on different 14S subunits during assembly of 14S into 70S particles. Both 14S and 70S particles elicited HAV-neutralizing antibodies in mice; thus, these particles may be useful for HAV vaccine development.
甲型肝炎病毒（HAV）有一个免疫显性中和抗原位点。通过使用一组针对HAV中和抗原位点的单克隆抗体，研究表明该位点内的三个表位存在于14S亚基（结构单元的五聚体）上。相比之下，该位点内的另外两个表位是在14S亚基组装成衣壳时形成的。因此，这两种单克隆抗体识别的表位要么是由抗原位点的构象变化形成的，要么是在14S组装成70S颗粒的过程中，不同14S亚基上存在的表位片段并列形成的。14S和70S颗粒均可在小鼠体内引发HAV中和抗体；因此，这些颗粒可能对HAV疫苗的开发有用。
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