Fluorescence staining of nuclear deoxyribonucleic acid allows for accurate assessment of the hamster egg penetration assay.
作者信息
el-Danasouri I, Milki A, Gebhardt J, Louie D, Westphal L
机构信息
Department of Gynecology and Obstetrics, Stanford University School of Medicine, Stanford, California.
出版信息
Fertil Steril. 1993 Feb;59(2):470-2. doi: 10.1016/s0015-0282(16)55694-5.
PMID:7678824
Abstract
To improve the assessment of sperm penetration during the hamster penetration assay, we compared the Hoechst 33342 and 33258 DNA-specific fluorescent stains with the standard acetolacmoid stain. The fluorescence stains produced distinct staining of the DNA within the egg cytoplasm and nucleus, and this allowed for accurate and fast assessment of sperm penetration.
摘要
Zotero 插件