Mashimo H, Podolsky D K, Fishman M C
Gastrointestinal Unit, Massachusetts General Hospital, Boston 02114, USA.
Biochem Biophys Res Commun. 1995 May 5;210(1):31-7. doi: 10.1006/bbrc.1995.1623.
Intestinal Trefoil Factor (ITF) is a member of a family of gastrointestinal tract peptides with region-specific expression which are enhanced at sites of injury and repair. In the present study, the murine homologue gene of ITF was molecularly cloned in order to characterize the structure and expression of this peptide in mice. Murine ITF exhibited 78, 95 and 94% nucleotide homology respectively in exons I, II and III, with overall 90% predicted amino acid identity when compared to the rat ITF. Murine ITF exhibited 70% inferred amino acid identity compared with human ITF. Northern blot analysis of various adult mouse tissues demonstrated that ITF is expressed abundantly in the intestine and colon, and minimally in stomach, but not in brain, lung, spleen, kidney, uterus, pancreas, liver, heart or thymus tissues. Expression of ITF appeared to occur as a post-natal event: antibody specific for ITF stains intensely goblet cells in the intestine and colon of three-day old and older mice, but not in the gastrointestinal tract of younger mice or embryos.
肠三叶因子（ITF）是胃肠道肽家族的一员，具有区域特异性表达，在损伤和修复部位表达增强。在本研究中，对ITF的小鼠同源基因进行了分子克隆，以表征该肽在小鼠中的结构和表达。与大鼠ITF相比，小鼠ITF在外显子I、II和III中分别表现出78%、95%和94%的核苷酸同源性，预测氨基酸总体同一性为90%。与人类ITF相比，小鼠ITF的推断氨基酸同一性为70%。对成年小鼠各种组织的Northern印迹分析表明，ITF在小肠和结肠中大量表达，在胃中表达最少，但在脑、肺、脾、肾、子宫、胰腺、肝脏、心脏或胸腺组织中不表达。ITF的表达似乎是出生后的事件：针对ITF的抗体强烈染色3日龄及以上小鼠小肠和结肠中的杯状细胞，但不染色较年幼小鼠或胚胎的胃肠道。
