Romagnoli A, Oradei A, Destito C, Iacocagni A, Marin A W, Littarru G P
Institute of Clinical Surgery, Catholic University of Sacred Heart, Rome, Italy.
Mol Aspects Med. 1994;15 Suppl:s177-85. doi: 10.1016/0098-2997(94)90027-2.
The authors prepared an experimental animal model of ischemia and reperfusion of the limbs to evaluate in vivo the reactive oxygen species involvement and protective role of coenzyme Q10 in reperfusion injury. A group of male rabbits (untreated group) underwent clamping of abdominal aorta for 3 hr and then declamping; at intervals blood sampling was drawn for coenzyme Q10, vitamin E, lactic acid and creatine kinase assays. Another group of male rabbits (treated group) underwent the same ischemia period but before declamping coenzyme Q10 was administered intra aorta. In untreated group, coenzyme Q10 and vitamin E plasma levels decreased while lactic acid and creatine kinase plasma levels increased during reperfusion. These data demonstrate that, after only 3 hr of ischemia, the extremities show a biochemical reperfusion injury, and this involves an increased consumption of antioxidants such as coenzyme Q10 and vitamin E. In the treated group, the increase of creatine kinase plasma levels during reperfusion was not significant, while the decrease in vitamin E was more marked.
作者制备了肢体缺血再灌注的实验动物模型，以在体内评估活性氧的参与情况以及辅酶Q10在再灌注损伤中的保护作用。一组雄性兔子（未治疗组）进行腹主动脉夹闭3小时，然后解除夹闭；每隔一段时间采集血样，进行辅酶Q10、维生素E、乳酸和肌酸激酶检测。另一组雄性兔子（治疗组）经历相同的缺血期，但在解除夹闭前经主动脉内给予辅酶Q10。在未治疗组中，再灌注期间血浆辅酶Q10和维生素E水平下降，而血浆乳酸和肌酸激酶水平升高。这些数据表明，仅缺血3小时后，肢体就出现了生化再灌注损伤，这涉及抗氧化剂如辅酶Q10和维生素E的消耗增加。在治疗组中，再灌注期间血浆肌酸激酶水平的升高不显著，而维生素E的下降更为明显。
