来自诺里病（NDP）基因座的双向酵母人工染色体步移。

A bidirectional YAC walk from the Norrie disease (NDP) locus.

作者信息

Black G C, Coleman M P, Chen Z Y, Nemeth A H, Davies K E, Craig I W

机构信息

Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, United Kingdom.

出版信息

Genomics. 1995 Feb 10;25(3):644-9. doi: 10.1016/0888-7543(95)80006-8.

DOI:10.1016/0888-7543(95)80006-8

PMID:7759098

Abstract

The region of Xp between DXS7 and the centromere contains the gene for Norrie disease in addition to the genes for several other ophthalmic disorders. A 650-kb YAC containing the loci MAOA, MAOB, and NDP has been used as the starting point for a bidirectional chromosomal walk. A contig of 16 YACs covering between 2 and 3 Mb has been developed in which the following markers/genes are located (in physical order): Xpter--DXS1201 (256ze5)--DXS6668--DXS228--DXS77--MAOA--++ +MAOB--FR12 (pseudogene)--NDP--DXS6670--RRM2P3--DXS6671--DXS742 --Xcen. Seven new STSs are described both for end clones and for internal Alu PCR products from the contig. The contig contains the breakpoint of the t75-2ma-1b (t75) translocation, close to the 5' end of the MAOB gene.

摘要

在DXS7和着丝粒之间的Xp区域，除了包含几种其他眼科疾病的基因外，还包含诺里病基因。一个包含MAOA、MAOB和NDP基因座的650 kb酵母人工染色体（YAC）已被用作双向染色体步移的起点。现已构建了一个由16个YAC组成的重叠群，覆盖2至3 Mb，其中包含以下按物理顺序排列的标记/基因：Xp末端——DXS1201（256ze5）——DXS6668——DXS228——DXS77——MAOA——++ +MAOB——FR12（假基因）——NDP——DXS6670——RRM2P3——DXS6671——DXS742 ——X着丝粒。本文描述了7个新的序列标签位点（STS），分别用于重叠群的末端克隆和内部Alu聚合酶链反应（PCR）产物。该重叠群包含t75 - 2ma - 1b（t75）易位的断点，靠近MAOB基因的5'端。