Chase H A
Department of Chemical Engineering, University of Cambridge, UK.
Trends Biotechnol. 1994 Aug;12(8):296-303. doi: 10.1016/0167-7799(94)90046-9.
Adsorption in stable expanded beds enables proteins to be recovered directly from particulate-containing feedstocks, such as fermentation broths and preparations of disrupted cells, without the need for prior removal of the suspended solids, which would normally result in the blockage of packed beds. The adoption of this technique will greatly reduce the complexity of downstream processing by eliminating certain filtration, centrifugation and concentration steps. Factors that are critical to the success of the procedure include the correct choice of adsorbent, together with careful design of the apparatus in which the separation is performed. The design, optimization and scale-up of appropriate operating protocols for expanded-bed procedures are very similar to those used for the operation of packed beds.
稳定膨胀床中的吸附作用能使蛋白质直接从含颗粒的原料（如发酵液和破碎细胞制剂）中回收，而无需事先去除悬浮固体，否则通常会导致填充床堵塞。采用该技术将通过省去某些过滤、离心和浓缩步骤大大降低下游加工的复杂性。该过程成功的关键因素包括吸附剂的正确选择以及对进行分离的设备的精心设计。膨胀床操作合适的操作方案的设计、优化和放大与填充床操作所使用的方案非常相似。
