Stokke T, Collins C, Kuo W L, Kowbel D, Shadravan F, Tanner M, Kallioniemi A, Kallioniemi O P, Pinkel D, Deaven L
University of California, San Francisco, USA.
Genomics. 1995 Mar 1;26(1):134-7. doi: 10.1016/0888-7543(95)80092-z.
The physical locations of 46 cosmid clones and 21 P1 clones were determined along the chromosome 20 axis relative to the p terminus (FLpter) using fluorescence in situ hybridization (FISH) and digital image microscopy. The cosmid clones were selected from the chromosomally enriched library LA20NC01. Nine P1 clones were selected from a pooled DuPont genomic library using PCR with primer pairs selected to amplify genetically mapped sequence-tagged sites. This information was used to relate the physical map to the genetic map. Twelve P1 clones were selected from the same library using PCR primer pairs that amplified known genes. Two of these, E2F and BCLX, had not been mapped previously.
利用荧光原位杂交（FISH）和数字图像显微镜技术，确定了46个黏粒克隆和21个P1克隆相对于20号染色体p端（FLpter）在染色体轴上的物理位置。黏粒克隆是从染色体富集文库LA20NC01中挑选出来的。9个P1克隆是从杜邦基因组文库池中通过PCR选出的，所用引物对用于扩增遗传定位的序列标签位点。该信息用于将物理图谱与遗传图谱相关联。另外12个P1克隆是从同一文库中通过能扩增已知基因的PCR引物对选出的。其中两个，即E2F和BCLX，此前尚未定位。
