作为合成肽和大肠杆菌融合蛋白表达的猫传染性腹膜炎病毒刺突基因区域的血清学识别
Serological recognition of feline infectious peritonitis virus spike gene regions expressed as synthetic peptides and E. coli fusion protein.
作者信息
Suiter B T, Pfeiffer N E, Jones E V, Reed A P, Klepfer S R, Miller T J, Srikumaran S
机构信息
SmithKline Beecham Animal Health, Lincoln, Nebraska, USA.
出版信息
Arch Virol. 1995;140(4):687-702. doi: 10.1007/BF01309958.
Cats exposed to feline infectious peritonitis virus (FIPV) or feline enteric coronavirus (FECV) cannot be differentiated by serological analysis. Three synthetic peptides and an E. coli recombinant fusion protein generated from FIPV 79-1146 spike gene sequence were produced. Coronavirus positive cat sera reacted to peptide aa 950-990 but were non-reactive to aa137-151 and aa 150-180 peptides as demonstrated by ELISA. Amino acid sequence 97-222 expressed as a galk fusion protein in E. coli was tested against coronavirus positive cat sera by western blot analysis. Only sera from cats exposed to the FIPV type-II strains DF-2 or 79-1146 that were exhibiting signs of FIP recognized the fusion protein. Sera from FECV exposed cats did not recognize the 97-222 fusion protein in western blot analysis.
暴露于猫传染性腹膜炎病毒（FIPV）或猫肠道冠状病毒（FECV）的猫无法通过血清学分析进行区分。我们制备了三种合成肽以及一种由FIPV 79 - 1146刺突基因序列产生的大肠杆菌重组融合蛋白。通过酶联免疫吸附测定（ELISA）证明，冠状病毒阳性猫血清与950 - 990氨基酸肽发生反应，但与137 - 151氨基酸肽和150 - 180氨基酸肽无反应。通过蛋白质印迹分析，检测了在大肠杆菌中作为半乳糖激酶（galK）融合蛋白表达的97 - 222氨基酸序列与冠状病毒阳性猫血清的反应情况。只有暴露于表现出猫传染性腹膜炎症状的II型FIPV毒株DF - 2或79 - 1146的猫的血清能识别该融合蛋白。在蛋白质印迹分析中，暴露于FECV的猫的血清不能识别97 - 222融合蛋白。
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